Phytochemical Screening and Antibacterial Activity of Sirih Leaves (Piper betle. Linn)

  • Ropisah Me
  • Nurul Aida Sarol
  • Nursyahirah Syahidah Baharim


Sirih with its scientific name Piper betle Linn is one of the most valuable herbs from Piperaceae family. Therefore, this study was focused on examining aimed the extraction of Sirih leaves, to determine the compounds in the extracted sample and investigate their antibacterial features. The grinded Sirih leaves (Piper betle Linn) were extracted by using two different polarity of solvent namely chloroform and ethanol by using hot extraction method. The percentage yield obtained for ethanol extract was 13.10%, higher as compared to the percentage yield of chloroform extract, which was 5.32%. The ethanol extract has indicated the presence of most phytochemicals such as flavonoid, tannin, phlobatannin, terpenoid, alkaloid and phenolic compounds, with no saponin. Meanwhile, chloroform extract showed only the presence of saponin compound. Each of the crudes was also investigated for their biological activities towards four different bacteria including Salmonella typhi, Escherichia coli, Staphylococcus aureus and Bacillus subtilis by using the disc diffusion method. The result reveals that ethanol extract of Sirih leaves have high potential for being antibacterial agent.

How to Cite
ME, Ropisah; SAROL, Nurul Aida; BAHARIM, Nursyahirah Syahidah. Phytochemical Screening and Antibacterial Activity of Sirih Leaves (Piper betle. Linn). Asian Journal of Fundamental and Applied Sciences, [S.l.], v. 2, n. 2, p. 15-23, june 2021. Available at: <>. Date accessed: 24 oct. 2021.