In Vitro Anti-Collagenase Activity and Total Phenolic Content Of Five Selected Herbs: A Review

  • Siti Maisarah Shukri Faculty of Applied Sciences, Universiti Teknologi MARA, 40450 Shah Alam, Selangor, Malaysia
  • Faezah Pardi 1Faculty of Applied Sciences, Universiti Teknologi MARA (UiTM), 40450 Shah Alam, Selangor, Malaysia 2Institute for Biodiversity and Sustainable Development, Office of Deputy Vice-Chancellor (Research & Innovation), 40450 Shah Alam, Selangor, Malaysia
  • Norrizah Jaafar Sidik Faculty of Applied Sciences, Universiti Teknologi MARA, 40450 Shah Alam, Selangor, Malaysia


Etlingera elatior, Persicaria odorata, Centella asiatica, Senna alata and Phyllanthus emblica were reviewed for their in vitro anti-collagenase activity and its total phenolic contents. Plant herbs play a vital role to promote the production of collagen which is the component of the skin. Different solvents during plant extraction and various plant parts were used to determine anti-collagenase properties and total phenolic content. Generally, anti-collagenase assay and Folin-Ciocalteu method were used to determine the anti-collagenase activity and total phenolic content, respectively. The extracts of these herbs have the potential of inhibiting collagenase activity and they possess total phenolic content. The total phenolic was found to be important elements in collagenase inhibition activity other than flavonoid. The selected herbs are popular in Malaysia and this review can be a useful reference or information for future application in pharmaceutical, food and cosmetics fields.

Keywords: Anti-collagenase activity, total phenolic contents; plant Extraction; flavonoids


[1] Apraj, V. D., & Pandita, N. S. (2016). Evaluation of skin anti-aging potential of Citrus reticulata blanco peel. Pharmacognosy research, 8(3), 160.
[2] Toriseva, M. J., Ala-aho, R., Karvinen, J., Baker, A. H., Marjomäki, V. S., Heino, J., & Kähäri, V. M. (2007). Collagenase-3 (MMP-13) enhances remodeling of three-dimensional collagen and promotes survival of human skin fibroblasts. Journal of investigative dermatology, 127(1), 49-59.
[3] Dash, G. K., & Zakaria, Z. B. (2016). Pharmacognostic studies on Persicaria odorata (Lour.) Sojak. Journal of Pharmacy Research, 10(6), 377-380.
[4] Wang, X., & Wu, J. (2019). Modulating effect of fatty acids and sterols on skin aging. Journal of Functional Foods, 57, 135-140.
[5] Madan, K., & Nanda, S. (2018). In-vitro evaluation of antioxidant, anti-elastase, anti-collagenase, anti-hyaluronidase activities of safranal and determination of its sun protection factor in skin photoaging. Bioorganic Chemistry, 77, 159-167.
[6] Kim Sooi, L., & Lean Keng, S. (2013). Herbal medicines: Malaysian women’s knowledge and practice. Evidence-based complementary and alternative medicine, 2013.
[7] Whangsomnuek, N., Mungmai, L., Mengamphan, K., & Amornlerdpison, D. (2019). Bioactive compounds of aqueous extracts of flower and leaf of Etlingera elatior (Jack) RM Sm. for cosmetic application. Maejo International Journal of Science and Technology, 13(3), 196-208.
[8] Whangsomnuek, N., Mungmai, L., Mengamphan, K., & Amornlerdpison, D. (2019). Efficiency of Skin Whitening Cream Containing Etlingera elatior Flower and Leaf Extracts in Volunteers. Cosmetics, 6(3), 39.
[9] Ridzuan, P. M., Hairul Aini, H., Norazian, M. H., Shah, A., Roesnita, & Aminah, K. S. (2013). Antibacterial and Antifungal Properties of Persicaria odorata Leaf Against Pathogenic Bacteria and Fungi. The Open Conference Proceedings Journal, 4, 71-74.
[10] Shavandi, M. A., Haddadian, Z., & Ismail, M. H. S. (2012). Eryngium foetidum L. Coriandrum sativum and Persicaria odorata L.: a review. Journal of Asian Scientific Research, 2(8), 410.
[11] James, J. T., & Dubery, I. A. (2009). Pentacyclic triterpenoids from the medicinal herb, Centella asiatica (L.) Urban. Molecules, 14(10), 3922-3941.
[12] Arora, R., Kumar, R., Agarwal, A., Reeta, K. H., & Gupta, Y. K. (2018). Comparison of three different extracts of Centella asiatica for anti-amnesic, antioxidant and anticholinergic activities: in vitro and in vivo study. Biomedicine & Pharmacotherapy, 105, 1344-1352.
[13] Saidina, S. H., Alia, N. A. M., Mohd, N., Hirmizia, N. Y., Abdullahb, Z., Markandanc, S., ... & Hashimb, N. (2019). Skin Care Active Ingredients from Senna alata (L.) Roxb Extracts. Asian J. Pharmacogn, 3(1), 23-31.
[14] Owoyale, J. A., Olatunji, G. A., & Oguntoye, S. O. (2005). Antifungal and antibacterial activities of an alcoholic extract of Senna alata leaves. Journal of Applied Sciences and Environmental Management, 9(3), 105-107.
[15] Sule, W. F., Okonko, I. O., Omo-Ogun, S., Nwanze, J. C., Ojezele, M. O., Ojezele, O. J., ... & Olaonipekun, T. O. (2011). Phytochemical properties and in-vitro antifungal activity of Senna alata Linn. crude stem bark extract. Journal of medicinal plants research, 5(2), 176-183.
[16] Liu, X., Cui, C., Zhao, M., Wang, J., Luo, W., Yang, B., & Jiang, Y. (2008). Identification of phenolics in the fruit of emblica (Phyllanthus emblica L.) and their antioxidant activities. Food chemistry, 109(4), 909-915.
[17] Van Wart, H. E., & Steinbrink, D. R. (1981). A continuous spectrophotometric assay for Clostridium histolyticum collagenase. Analytical biochemistry, 113(2), 356-365.
[18] Hussin, M., Abdul Hamid, A., Abas, F., Ramli, N. S., Jaafar, A. H., Roowi, S., ... & Pak Dek, M. S. (2019). NMR-based metabolomics profiling for radical scavenging and anti-aging properties of selected herbs. Molecules, 24(17), 3208.
[19] Thring, T. S., Hili, P., & Naughton, D. P. (2009). Anti-collagenase, anti-elastase and anti-oxidant activities of extracts from 21 plants. BMC complementary and alternative medicine, 9(1), 27.
[20] Chatatikun, M., & Chiabchalard, A. (2017). Thai plants with high antioxidant levels, free radical scavenging activity, anti-tyrosinase and anti-collagenase activity. BMC complementary and alternative medicine, 17(1), 487.
[21] Weingarten, H., & Feder, J. (1985). Spectrophotometric assay for vertebrate collagenase. Analytical biochemistry, 147(2), 437-440.
[22] Nithitanakool, S., Teeranachaideekul, V., Ponpanich, L., Nopporn, N., Junhunkit, T., Wanasawas, P., & Chulasiri, M. (2014). In vitro and in vivo skin whitening and anti-aging potentials of hydroglycolic extract from inflorescence of Etlingera elatior. Journal of Asian Association of Schools of Pharmacy, 3, 314-325.
[23] Pientaweeratch, S., Panapisal, V., & Tansirikongkol, A. (2016). Antioxidant, anti-collagenase and anti-elastase activities of Phyllanthus emblica, Manilkara zapota and silymarin: An in vitro comparative study for anti-aging applications. Pharmaceutical biology, 54(9), 1865-1872.
[24] Madhan, B., Krishnamoorthy, G., Rao, J. R., & Nair, B. U. (2007). Role of green tea polyphenols in the inhibition of collagenolytic activity by collagenase. International journal of biological macromolecules, 41(1), 16-22.
[25] Martins, F. S., Borges, L. L., Paula, J. R., & Conceição, E. C. (2013). Impact of different extraction methods on the quality of Dipteryx alata extracts. Revista Brasileira de Farmacognosia, 23(3), 521-526.
[26] Zhang, Q. W., Lin, L. G., & Ye, W. C. (2018). Techniques for extraction and isolation of natural products: a comprehensive review. Chinese medicine, 13(1), 20.
[27] George, A. G. V., & Yee, K. M. (2018). U.S. Patent No. 9,877,993. Washington, DC: U.S. Patent and Trademark Office.
[28] Johari, M. A., & Khong, H. Y. (2019). Total phenolic content and antioxidant and antibacterial activities of Pereskia bleo. Advances in Pharmacological and Pharmaceutical Sciences, 2019.
[29] Gan, R. Y., Chan, C. L., Yang, Q. Q., Li, H. B., Zhang, D., Ge, Y. Y., ... & Corke, H. (2019). Bioactive compounds and beneficial functions of sprouted grains. In Sprouted grains (pp. 191-246). AACC International Press.
[30] Wittenauer, J., Mäckle, S., Sußmann, D., Schweiggert-Weisz, U., & Carle, R. (2015). Inhibitory effects of polyphenols from grape pomace extract on collagenase and elastase activity. Fitoterapia, 101, 179-187.
[31] Mashkor, A. L. (2014). Phenolic content and antioxidant activity of fenugreek seeds extract. International Journal of Pharmacognosy and Phytochemical Research, 6(4), 841-844.
[32] Areekul, V., & Phomkaivon, N. (2015). Thai indigenous plants: focusing on Total phenolic content, antioxidant activity and their correlation on medicinal effects. Current Applied Science and Technology, 15(1), 10-23.
[33] Kupina, S., Fields, C., Roman, M. C., & Brunelle, S. L. (2019). Determination of Total Phenolic Content Using the Folin-C Assay: Single-Laboratory Validation, First Action 2017.13. Journal of AOAC International, 102(1), 320-321.
[34] Fitriansyah, S. N., Aulifa, D. L., Febriani, Y., & Sapitri, E. (2018). Correlation of total phenolic, flavonoid and carotenoid content of Phyllanthus emblica extract from Bandung with DPPH scavenging activities. Pharmacognosy Journal, 10(3).
[35] Sabaragamuwa, R., Perera, C. O., & Fedrizzi, B. (2018). Centella asiatica (Gotu kola) as a neuroprotectant and its potential role in healthy ageing. Trends in Food Science & Technology, 79, 88-97.
[36] Juwita, T., Puspitasari, I. M., & Levita, J. (2018). Torch ginger (Etlingera elatior): A review on its botanical aspects, phytoconstituents and pharmacological activities. Pak. J. Biol. Sci, 21(4), 151-165.
[37] Szewczyk, K., Miazga-Karska, M., Pietrzak, W., Komsta, Ł., Krzemińska, B., & Grzywa-Celińska, A. (2020). Phenolic composition and skin-related properties of the aerial parts extract of different Hemerocallis cultivars. Antioxidants, 9(8), 690.
How to Cite
SHUKRI, Siti Maisarah; PARDI, Faezah; JAAFAR SIDIK, Norrizah. In Vitro Anti-Collagenase Activity and Total Phenolic Content Of Five Selected Herbs: A Review. Science Letters, [S.l.], v. 15, n. 1, p. 117-127, jan. 2021. ISSN 2682-8626. Available at: <>. Date accessed: 07 feb. 2023. doi:
Articles Normal Issue

Most read articles by the same author(s)

Obs.: This plugin requires at least one statistics/report plugin to be enabled. If your statistics plugins provide more than one metric then please also select a main metric on the admin's site settings page and/or on the journal manager's settings pages.