Prevalence of Escherichia Coli from Raw Fresh Milk Produced from Farm in Negeri Sembilan
Milk is an extremely nutritious source of food as it contains colloidal suspension of protein, fat, carbohydrate, vitamin and mineral. Drinking raw or undercook milk may harbor the most common foodborne pathogenic bacteria if prepared inexpertly. The aim of this study was to detect the presence and susceptibility level of Escherichia coli towards selected antibiotics in cow raw fresh milk produced on dairy farms in Negeri Sembilan. The cow raw fresh milk samples were randomly selected from Pusat Perkhidmatan Industri Tenusu (PPIT) Kuala Pilah, Negeri Sembilan. Escherichia coli were isolated on Eosin Methylene Blue agar (EMBA) and confirmation was performed by using a series of biochemical test. The resuts revealed that only six out of nine isolates were positive for Escherichia coli, which exhibits a high resistance (100%) towards Vancomycin (VA5). In fact, none of the Escherichia coli isolates were resistant towards Gentamicin (CN10) and Streptomycin (S10). In addition, the multiple antibiotics resistance (MAR) indices were arranged from 0.2 to 0.4. The presence of Escherichia coli in the samples understudy had revealed that there was a high probability of contamination occurred due to the inadequate hygiene condition performed by the farmers while milking, storing and transporting the milk.